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Journal / Cancer Research / 荧光显微镜评价辐射诱发克隆细胞死亡模式的一步协议…
JoVE Journal
Cancer Research

This content is Open Access.

JoVE Journal Cancer Research
荧光显微镜评价辐射诱发克隆细胞死亡模式的一步协议
High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines
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High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines
Capture and Release of Viable Circulating Tumor Cells from Blood
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Capture and Release of Viable Circulating Tumor Cells from Blood
 
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荧光显微镜评价辐射诱发克隆细胞死亡模式的一步协议

Article DOI: 10.3791/56338-v • 06:47 min • October 23rd, 2017
October 23rd, 2017 •
1, 2, 1, 1
1Department of Radiation Oncology, Gunma University Graduate School of Medicine, 2Advanced Scientific Research Leaders Development Unit, Gunma University Graduate School of Medicine

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电离辐射 (ir) 诱导的克隆细胞死亡的研究对于了解 ir 对恶性肿瘤和正常组织的影响是非常重要的。在这里, 我们描述了基于 4 ', 6-diamidino-2-吲二盐酸盐 (DAPI)-荧光显微镜可视化染色核的形态学的红外诱导克隆细胞死亡主要模式的 one-step 测定。

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癌症研究 问题 128 辐射生物学 电离辐射 克隆细胞死亡 凋亡 有丝分裂突变 细胞衰老 荧光显微镜 DAPI 染色
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