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Journal
/
Biology
/
检测莱姆病螺旋体,
螺旋体螺旋体
, 在蜱中使用巢式 PCR
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal
Biology
Detecting the Lyme Disease Spirochete,
Borrelia Burgdorferi
, in Ticks Using Nested PCR
Please note that all translations are automatically generated.
Click here for the English version.
检测莱姆病螺旋体,
螺旋体螺旋体
, 在蜱中使用巢式 PCR
DOI:
10.3791/56471-v
•
07:20 min
•
February 04, 2018
•
Melanie K. B. Wills
,
Andrea M. Kirby
,
Vett K. Lloyd
1
Department of Biology
,
Mount Allison University
Chapters
00:00
Title
01:42
DNA Isolation from Ticks
04:06
Nested PCR Detection of
Borrelia OspA
and
FlaB
05:25
Results
06:22
Conclusions
Summary
Automatic Translation
English (Original)
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Automatic Translation
巢式 PCR 是一种灵敏的、特异的、直接的技术, 可以应用于蜱 DNA 提取物来探测莱姆病的致病剂
螺旋体螺旋体
。最初的 PCR 实验使用基因特异的引物产生长的增, 然后成为模板为随后反应使用内部引物。
Tags
Lyme Disease
Borrelia Burgdorferi
Tick
Nested PCR
Pathogen Detection
DNA Extraction
Molecular Surveillance
Article
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