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高解像度光学マッピングとマウスの心房の電気生理学的評価
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Electrophysiological Assessment of Murine Atria with High-Resolution Optical Mapping
Please note that all translations are automatically generated.
Click here for the English version.
高解像度光学マッピングとマウスの心房の電気生理学的評価
DOI:
10.3791/56478-v
•
08:19 min
•
February 22, 2018
•
Kensuke Ihara*
1
,
Koji Sugiyama*
1
,
Kentaro Takahashi*
1
,
Masahiro Yamazoe
,
Tetsuo Sasano
,
Tetsushi Furukawa
1
Department of Bio-informational Pharmacology, Medical Research Institute
,
Tokyo Medical and Dental University
,
2
Department of Biofunctional Informatics
,
Tokyo Medical and Dental University
Chapters
00:04
Title
00:48
Mouse Cannulation and Perfusion for Optical Mapping using Langendorff
03:37
Staining for Single Recording Membrane Voltage, Burst Pacing, and Extra-stimulus Pacing
06:37
Results: Conduction Patterns during Normal Sinus Rhythm and Atrial Tachyarrhythmia
07:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
このプロトコルを記述する高時空分解能、膜電位のデュアル録音を含む光学マッピング システムを利用したマウスの心房の電気生理学的評価と Ca
2 +
の下で一時的なプログラム特殊な電極カテーテルを刺激。
Tags
Electrophysiological Assessment
Murine Atria
High-resolution Optical Mapping
Cardiac Electrophysiology
Atrial Arrhythmia
Langendorff Apparatus
Perfusion Circuit
Anesthesia
Heart Excision
Tyrode’s Solution
Perfusion Pressure
Vena Cava
Glass Chamber
Ventricular Cavity
Stimulation Electrode
Electrocardiogram
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