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SwarmSight: Real-time Tracking of Insect Antenna Movements and Proboscis Extension Reflex Using a Common Preparation and Conventional Hardware
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
SwarmSight: Real-time Tracking of Insect Antenna Movements and Proboscis Extension Reflex Using a Common Preparation and Conventional Hardware

SwarmSight: Real-time Tracking of Insect Antenna Movements and Proboscis Extension Reflex Using a Common Preparation and Conventional Hardware

DOI:
10.3791/56803-v

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08:13 min

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December 25, 2017

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, , , ,
1School of Life Sciences,Arizona State University, 2School of Mathematical and Statistical Sciences,Arizona State University

Chapters

  • 00:05Title
  • 00:58Catching and Harnessing Honey Bees and Preparation of the Video Camera
  • 03:17Positioning Sensors and Video Analysis
  • 04:25Adjusting Filter Thresholds and Saving Results
  • 05:31Results: Appendage Tracking Software Demonstrates Higher Efficiency and Precision Compared to Human Raters
  • 07:05Conclusion

Summary

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This protocol describes steps for using the novel software, SwarmSight, for frame-by-frame tracking of insect antenna and proboscis positions from conventional web camera videos using conventional computers. The free, open-source software processes frames about 120 times faster than humans and performs at better than human accuracy.

Tags

Keywords: Insect AntennaeProboscis Extension ReflexReal-time TrackingVideo RecordingOpen-source SoftwareConventional HardwareHoney BeesOdor Response

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