Neuroscience
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Chapters
Summary September 24th, 2020
Animal brain functional mapping can benefit from the real-time functional magnetic resonance imaging (fMRI) experimental set-up. Using the latest software implemented in the animal MRI system, we established a real-time monitoring platform for small animal fMRI.
Transcript
Instead of retrospective analysis to investigate compounding sources. The proposed leader time fMRI platform provides a more effective scheme for identifying dynamic fMRI response using the latest console software. This technique can provide immediate troubleshooting feasibility and a real-time biofeedback simulation paradigm for brain functional studies in animals To acquire anatomical images covering the whole brain in the coronal view, click the continue button to run a T2 weighted rare sequence.
To set up the realtime fMRI software. Open a terminal window and use the command to navigate to the realtime AFNI plugin path. Use the commands as indicated to execute the AFNI software with the realtime plugin.
Use the diamond command and options to monitor the upcoming AFNI brick files. Use the PVC MD command with the options as indicated, to run the background macro script. To obtain the EPI acquisition parameters, use the exec PVC MD command and options.
To convert the 3D ECHO planar imaging raw data, to AFNI files, in real time, in the background macro script. Use the exec TOD-3D Command and options, making sure that EPI geometrical information is consistent with the anatomy orientation. Click the continue button to run a T2 Star Weighted 3D EPI Sequence for bold fMRI imaging.
To overlay the EPI images, onto the anatomical rare images. First, use the command to convert the rare images to an AFNI Brick dataset and use the script and options to register the EPI images to the anatomical images. To process resting-state functional connectivity maps of the bold responses.
Use AFNI options to calculate the seed based correlation of the 3D plus time dataset, such as resting state fMRI time series. Then to visualize functional maps of the bold signals, select the defined overlay option and use the clusters function, from the AFNI user interface menu, to generate an interactive clustering in the AFNI software. To visualize response electrical stimulation responses by fMRI.
Turn on an electrical stimulus isolator and use stimulation blocks to perform electrical four-paw stimulation for one evoked fMRI analysis. In this Representative Real-Time Voxel-Wise Bold fMRI Time Course. The voxel-wise time course of the contralaterally primary four-paw somatosensory cortex, corresponding to the block design paradigm in the real-time acquisition format, after electrical four-paw stimulation can be observed.
As illustrated activated bold maps corresponding to the electrical four-paw stimulation can also be generated with the activated regions detected and displayed as colored clusters. Be sure to activate the 3D EPI Reconstruction option pre-image 3D activity and execute macro in the data construction user interface menu. And to link to the predefining macro script set-up RT 3D API before scanning.
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