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Journal / Neuroscience / Simpel Generation af højtydende kultur af…
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Neuroscience

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JoVE Journal Neuroscience
Simpel Generation af højtydende kultur af inducerede neuroner fra menneskelige voksen hud fibroblaster
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Simpel Generation af højtydende kultur af inducerede neuroner fra menneskelige voksen hud fibroblaster

Article DOI: 10.3791/56904-v • 09:07 min • February 5th, 2018
February 5th, 2018 •
1, 1, 1,2, 1, 1
1Department of Experimental Medical Science, Wallenberg Neuroscience Center, Division of Neurobiology and Lund Stem Cell Center, Lund University, 2John van Geest Centre for Brain Repair & Department of Neurology, Department of Clinical Neurosciences and Cambridge Stem Cell Institute, University of Cambridge

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Direkte neuronal omprogrammering genererer neuroner, der opretholder en alder af det udgangspunktet i somatiske celler. Her, beskriver vi en enkelt vektor-baseret metode til at generere induceret neuroner fra dermal fibroblaster fremstillet af voksne menneskelige donorer.

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Neurovidenskab sag 132 Induced neuroner voksen human dermal fibroblaster direkte neurale omprogrammering menneskelige neuroner FACS rensning lentiviral vektor
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