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Journal / Biology / Interactome-Seq: En protokoll for Domainome…
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JoVE Journal Biology
Interactome-Seq
Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
Journal (Biology)
Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
Examination of Drosophila Larval Tracheal Terminal Cells by Light Microscopy
Journal (Biology)
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Interactome-Seq: En protokoll for Domainome biblioteket konstruksjon, validering og utvalg av Phage Display og neste generasjons sekvensering

Article DOI: 10.3791/56981-v • 12:04 min • October 3rd, 2018
October 3rd, 2018 •
1, 2, 2, 3, 3, 3, 4, 1, 4, 5,6
1Department of Health Sciences, Università del Piemonte Orientale & IRCAD, Novara, Italy, 2Institute of Biomedical Technologies, National Research Council, Segrate, Milan, Italy, 3Institute of Biomedical Technologies, National Research Council, Bari, Italy, 4Department of Life Sciences, University of Trieste, Italy, 5Institute of Genetic and Biomedical Research, National Research Council, Rozzano, Milan, Italy, 6Humanitas Clinical and Research Center, Rozzano, Milan, Italy

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Protokollene beskrevet tillater bygging, karakterisering og utvalg (mot målet valgfrihet) av et "domainome" bibliotek laget av noen DNA-kilde. Dette oppnås ved en forskning rørledningen som kombinerer forskjellige teknologier: phage skjerm, folding journalist og neste generasjons sekvensering med en web-verktøy for dataanalyse.

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Biologi problemet 140 Phage skjerm neste generasjons sekvensering Interactome Protein domene web-verktøy folding reporter proteinstruktur.
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