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JoVE Journal
Biology

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胰腺胰岛与小鼠分离的简单高效协议
 
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胰腺胰岛与小鼠分离的简单高效协议

Article DOI: 10.3791/57048-v 06:56 min August 30th, 2019
August 30th, 2019

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

此胰岛分离方案描述了胶原酶注射以消化外分子组织的新途径,以及从小鼠中纯化胰岛的简化梯度程序。它涉及酶消化,梯度分离/纯化,和小岛手工采摘。成功的隔离可以产生250~350个高质量和全功能的小岛每只鼠标。

Tags

生物学, 问题 150, 胰岛, 胰腺, 胶原酶, 密度梯度, 胰岛素, 糖尿病, 小鼠
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