JoVE Journal
Biology
Biology
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Summary
Please note that all translations are automatically generated.
此胰岛分离方案描述了胶原酶注射以消化外分子组织的新途径,以及从小鼠中纯化胰岛的简化梯度程序。它涉及酶消化,梯度分离/纯化,和小岛手工采摘。成功的隔离可以产生250~350个高质量和全功能的小岛每只鼠标。