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JoVE Journal
Biology

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分离高质量鼠心房和心室肌细胞,同时测量 Ca2+ 瞬变和 L 型钙电流
 
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分离高质量鼠心房和心室肌细胞,同时测量 Ca2+ 瞬变和 L 型钙电流

Article DOI: 10.3791/61964-v 06:22 min November 3rd, 2020
November 3rd, 2020

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Summary

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小鼠模型允许研究心律失常发生的关键机制。为此,需要高质量的心肌细胞来进行膜片钳测量。这里描述了一种通过逆行基于酶的Langendorff灌注分离鼠心房和心室肌细胞的方法,该方法允许同时测量钙瞬变和L型钙电流。

Tags

本月在JoVE上,第165期,心律失常,鼠肌细胞分离,朗根多夫灌注,L型钙电流,钙瞬变,膜片钳
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