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JoVE Journal Neuroscience
Isolation og kultur af gnaver mikroglia at fremme en dynamisk forgrenet morfologi i serumfrit Medium
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Isolation og kultur af gnaver mikroglia at fremme en dynamisk forgrenet morfologi i serumfrit Medium

Article DOI: 10.3791/57122-v • 12:00 min • March 9th, 2018
March 9th, 2018 •
1, 1
1Department of Neurobiology, Stanford University

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Bestræbelser på at forstå microglial funktion i detaljer har været hæmmet af manglen på microglial kultur modeller, at sammenfatte egenskaberne af modne i vivo mikroglia. Denne protokol beskriver en isolation og kultur tilgang har til formål at fastholde robuste overlevelse af stærkt forgrenet modne rotte mikroglia defineret-medium betingelser.

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Neurovidenskab sag 133 mikroglia celle kultur definerede Medium neurovidenskab makrofag serumfrit forgrenet immunologi
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