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Forberedelse af Murine submandibulære spytkirtel til opretstående Intravital mikroskopi
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy
DOI:

08:13 min

May 07, 2018

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Chapters

  • 00:05Title
  • 00:39Removal of the Fur and Fixation of the Mouse on the Stage
  • 02:46Surgical Exposure of the Salivary Gland Using a Stereomicroscope
  • 04:09Fixing of the Gland, Heating Ring, and the Temperature Probe
  • 06:10Results: Use of Immunofluorescence Imaging to Study the Submandibular Gland
  • 07:19Conclusion

Summary

Automatic Translation

Vi beskriver en protokol for at kirurgisk afsløre og stabilisere den murine submandibulære spytkirtel for intravital billeddannelse ved hjælp af opretstående intravital mikroskopi. Denne protokol er let at tilpasse til andre eksokrine kirtler af hoved og hals region af mus og andre små gnavere.

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