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Utarbeidelse av Murine Submandibular Salivary kjortelen for stående Intravital mikroskopi
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy
DOI:

08:13 min

May 07, 2018

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Chapters

  • 00:05Title
  • 00:39Removal of the Fur and Fixation of the Mouse on the Stage
  • 02:46Surgical Exposure of the Salivary Gland Using a Stereomicroscope
  • 04:09Fixing of the Gland, Heating Ring, and the Temperature Probe
  • 06:10Results: Use of Immunofluorescence Imaging to Study the Submandibular Gland
  • 07:19Conclusion

Summary

Automatic Translation

Vi beskriver en protokoll for å kirurgisk avsløre og stabilisere det murine submandibular salivary kjortelen intravital bildevisning oppreist intravital mikroskopi. Denne protokollen er lett tilpasses andre exocrine kjertler av hodet og nakken regionen av mus og annet liten Red.

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