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Journal / Genetics / Generere transgene planter med enkelt-kopi…
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Genetics

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JoVE Journal Genetics
Generere transgene planter med enkelt-kopi innsettinger bruker BIBAC-GW binære vektor
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Generere transgene planter med enkelt-kopi innsettinger bruker BIBAC-GW binære vektor

Article DOI: 10.3791/57295-v • 12:08 min • March 28th, 2018
March 28th, 2018 •
1, 1, 1, 1, 1, 1, 1, 1
1Swammerdam Institute for Life Sciences, University of Amsterdam

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Bruke en pBIBAC-GW binære vektor gjør genererer transgene planter med intakt enkelt-kopi innsettinger, en enkel prosess. Her presenteres en rekke protokoller som veileder leseren gjennom prosessen med å generere transgene Arabidopsis planter og testing planter for intactness og kopierer antall skivene.

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Genetikk problemet 133 binære vektor BIBAC Gateway kompatibel binære vektor plante transformasjon enkel integrasjon gene stanse DNA blotting Southern blotting
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