Journal
/
Developmental Biology
/
In Vivo Imaging of Muscle-tendon Morphogenesis in Drosophila Pupae
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
In Vivo Imaging of Muscle-tendon Morphogenesis in Drosophila Pupae

In Vivo Imaging of Muscle-tendon Morphogenesis in Drosophila Pupae

DOI:
10.3791/57312-v

•

08:33 min

•

February 06, 2018

•

,
1Muscle Dynamics Group,Max Planck Institute of Biochemistry, 2Aix Marseille University,CNRS, IBDM

Chapters

  • 00:04Title
  • 00:31Stage Pupae
  • 01:45Prepare Pupae for Imaging
  • 03:31Mount Pupae
  • 05:58Live Imaging of Pupae
  • 06:33Results: Dramatic Morphological Changes Visible in Live Images
  • 08:06Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present an easy-to-use and versatile method to perform live imaging of developmental processes in general and muscle-tendon morphogenesis in particular in living Drosophila pupae.

Tags

Keywords: In Vivo ImagingMuscle-tendon MorphogenesisDrosophila PupaeMuscle DevelopmentTendon TargetsMuscle TwitchingFluorescent MarkersLive ImagingMuscle Biology

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57312?article_type=v

Upright Imaging of Drosophila Egg Chambers
check_url/57312?article_type=v

Imaging Calcium in Drosophila at Egg Activation
check_url/57312?article_type=v

Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta
check_url/57312?article_type=v

Live Imaging of Mouse Secondary Palate Fusion
check_url/57312?article_type=v

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging
check_url/57312?article_type=v

The C. elegans Excretory Canal as a Model for Intracellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis in a Single Cell: labeling by GFP-fusions, RNAi Interaction Screen and Imaging
check_url/57312?article_type=v

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains
check_url/57312?article_type=v

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
check_url/57312?article_type=v

Live Imaging and Analysis of Muscle Contractions in Drosophila Embryo
check_url/57312?article_type=v

Thawing, Culturing, and Cryopreserving Drosophila Cell Lines

Read Article