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Journal / Biochemistry / Titrering ELISA som en metod för att bestämma…
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Biochemistry

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JoVE Journal Biochemistry
Titrering ELISA som en metod för att bestämma konstanten Dissociation av Receptor Ligand interaktion
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Titrering ELISA som en metod för att bestämma konstanten Dissociation av Receptor Ligand interaktion

Article DOI: 10.3791/57334-v • 12:38 min • February 15th, 2018
February 15th, 2018 •
1
1Institute of Physiological Chemistry and Pathobiochemistry, University of Münster

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Ett detaljerat protokoll för att utföra en titrering ELISA beskrivs. Dessutom presenteras en ny algoritm att utvärdera titrering ELISA-test och få en dissociationskonstant bindning av ett lösligt ligand till en mikrotiter plattan-orörlig receptor.

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Biokemi fråga 132 ELISA titrering assay dissociation konstant icke-linjär regression receptor-ligand-interaktion rhodocetin
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