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Journal / Biochemistry / Cellulära membranaffinitetskromatografikolonner…
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Biochemistry

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JoVE Journal Biochemistry
Cellulära membranaffinitetskromatografikolonner för att identifiera specialiserade växtmetaboliter som interagerar med immobiliserad tromboyosinkinasreceptor B
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Cellulära membranaffinitetskromatografikolonner för att identifiera specialiserade växtmetaboliter som interagerar med immobiliserad tromboyosinkinasreceptor B

Article DOI: 10.3791/63118-v • 11:44 min • January 19th, 2022
January 19th, 2022 •
1,2, 1, 1, 1, 1
1Department of Biological Sciences, The University of Alabama, 2Department of Pharmaceutical Botany, Faculty of Pharmacy, Hacettepe University

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Protokollet beskriver beredningen av cellmembranaffinitetskromatografikolonner (CMAC) med immobiliserade cellmembranfragment innehållande funktionella transmembrantropomyosinkinasreceptor B-proteiner. Användningen av CMAC-kolumner vid identifiering av specialiserade växtmetaboliter som interagerar med dessa receptorer och närvarande i komplexa naturliga blandningar förklaras också.

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Biokemi utgåva 179
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