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Journal / Genetics / 一种简便、快速、定量的测定 DNA 蛋白 Crosslinks…
JoVE Journal
Genetics

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JoVE Journal Genetics
一种简便、快速、定量的测定 DNA 蛋白 Crosslinks 在转染哺乳动物细胞中的研究
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一种简便、快速、定量的测定 DNA 蛋白 Crosslinks 在转染哺乳动物细胞中的研究

Article DOI: 10.3791/57413-v • 11:58 min • March 5th, 2018
March 5th, 2018 •
1, 1
1Department of Pharmacology, University of Minnesota

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本协议的目的是量化在质粒 dna 上对定义的 dna 蛋白 crosslinks 的修复。损伤质粒被转染成受体哺乳动物细胞系和低分子量, 在多时间点后再染。DNA 修复动力学的量化使用链特定引伸后, qPCR。

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遗传学 问题 133 dna 蛋白 crosslinks dna 修复 定量聚合酶链反应 8 oxoguanine 人 oxoguanine glycosylase Taq 聚合酶
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