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Journal / Genetics / 使用DNA纤维测定检测人细胞中复制后间隙的积累和修复…
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Genetics

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JoVE Journal Genetics
使用DNA纤维测定检测人细胞中复制后间隙的积累和修复
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使用DNA纤维测定检测人细胞中复制后间隙的积累和修复

Article DOI: 10.3791/63448-v • 10:32 min • February 3rd, 2022
February 3rd, 2022 •
*1, *2, 2, 1
1Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, 2Division of Oncology, Department of Internal Medicine, Washington University in St. Louis
* These authors contributed equally

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在这里，我们描述了DNA纤维测定的两种修饰，以研究病变诱导后复制DNA的单链DNA间隙。S1纤维测定能够使用ssDNA特异性S1内切酶检测复制后间隙，而间隙填充测定允许间隙修复的可视化和定量。

Tags

遗传学，第180期，复制后单链DNA间隙，间隙填充，复制后修复，DNA复制，DNA纤维测定，S1核酸酶，再预切
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