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JoVE Journal
Immunology and Infection

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泡沫病毒 Intasomes 原型的组装与纯化
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泡沫病毒 Intasomes 原型的组装与纯化

Article DOI: 10.3791/57453-v 10:20 min March 19th, 2018
March 19th, 2018



Please note that all translations are automatically generated.

Click here for the English version.

重组逆转录病毒整合酶和 dna 寡聚物模仿病毒 dna 末端可以形成一个酶活跃的复杂称为 intasome。Intasomes 可用于生物化学、结构和动力学研究。本协议详细介绍了如何组装和纯化原型泡沫病毒 intasomes。


免疫学和感染 133 逆转录病毒 原型泡沫病毒 Intasome 整合酶 蛋白质复合体组装 蛋白质复合纯化
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