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JoVE Journal
Immunology and Infection

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流感病毒从感染细胞的染色质的核糖核蛋白复合物的亲和纯化
 
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流感病毒从感染细胞的染色质的核糖核蛋白复合物的亲和纯化

Article DOI: 10.3791/4028-v 11:20 min June 3rd, 2012
June 3rd, 2012

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

流感病毒与宿主细胞染色体复制他们在协会的RNA基因组。在这里,我们提出了一个方法来净化完整的病毒核蛋白复合物,从感染细胞的染色质。纯化病毒复合物,可以分析,Western blot和引物蛋白质和RNA含量的扩展,分别。

Tags

病毒学,64期,分子生物学,A型流感病毒,亲和纯化,亚细胞分离,染色,vRNP复合物,聚合酶
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