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重组原型泡沫病毒整合纯化过程中核酸污染的检测与去除
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Immunology and Infection
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JoVE Journal Immunology and Infection
Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase
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重组原型泡沫病毒整合纯化过程中核酸污染的检测与去除

DOI:
10.3791/56605-v

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07:50 min

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December 08, 2017

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, , ,
1Department of Cancer Biology and Genetics,The Ohio State University College of Medicine

Chapters

  • 00:05Title
  • 00:35Nickel Affinity Chromatography
  • 03:30Heparin Affinity Chromatography
  • 04:28Nuclease Assay
  • 06:46Results: Nuclease Assay of Heparin Sepharose Fractions
  • 07:19Conclusion

Summary

Automatic Translation

Automatic Translation

重组原型泡沫病毒整合蛋白经常被细菌核酸在纯化过程中污染。这种方法识别核酸污染, 并将其从酶的最终制备中除去。

Tags

Nuclease ContaminationProtein PurificationRecombinant Prototype Foamy Virus IntegraseE. ColiSonicationUltracentrifugationFPLCNickel-charged ResinSDS-PAGEUV Absorbance

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