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JoVE Journal
Bioengineering

This content is Open Access.

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
 

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Article doi: 10.3791/57551
May 4th, 2018 Usage Statistics

Summary May 4th, 2018

The protocol presents the Escherichia coli-based selective pressure incorporation of non-canonical amino acids (ncAAs) into the lactococcal antimicrobial peptide nisin. Its properties can be changed during recombinant expression via substitution with desired ncAAs in defined growth media. Resulting changes in bioactivity are mapped by growth inhibition assays and fluorescence microscopy.

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