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JoVE Journal Neuroscience
Enkelt celle Multiplex Reverse transkription Polymerase Chain Reaction efter Patch-klemme
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Enkelt celle Multiplex Reverse transkription Polymerase Chain Reaction efter Patch-klemme

Article DOI: 10.3791/57627-v • 10:44 min • June 20th, 2018
June 20th, 2018 •
*1, *1, *1, 1
1UPMC Univ Paris 06, INSERM, CNRS, Neuroscience Paris Seine - Institut de Biologie Paris Seine (NPS - IBPS), Sorbonne Université
* These authors contributed equally

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Denne protokol beskriver de kritiske trin og forholdsregler, der kræves for at udføre enkelt celle multiplex reverse transkription polymerase kædereaktion efter patch-klemme. Denne teknik er en enkel og effektiv metode til at analysere udtryk profil af en på forhånd fastlagt sæt af gener fra en enkelt celle karakteriseret ved patch-clamp optagelser.

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Neurovidenskab sag 136 Gene expression profil neuroner astrocytter hjernen skiver hele-celle konfiguration indlejrede Polymerasekædereaktionen (PCR)
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