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Journal / Biochemistry / Identifikation af Cyclin-afhængig Kinase 1…
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Biochemistry

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JoVE Journal Biochemistry
Identifikation af Cyclin-afhængig Kinase 1 specifikke fosforylering websteder ved en In Vitro -Kinase Assay
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Identifikation af Cyclin-afhængig Kinase 1 specifikke fosforylering websteder ved en In Vitro -Kinase Assay

Article DOI: 10.3791/57674-v • 12:26 min • May 3rd, 2018
May 3rd, 2018 •
*1, *1, 1, 1
1Department of Chemistry, State University of New York at Binghamton
* These authors contributed equally

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Cyclin-afhængig kinase 1 (Cdk1) er aktiveret i G2 fase af cellecyklus og regulerer mange cellulære veje. Her præsenterer vi en protokol for en in vitro- kinase assay med Cdk1, som giver mulighed for identifikation af Cdk1-specifikke fosforylering websteder til oprettelse af cellulære mål for denne vigtige kinase.

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Biokemi spørgsmålet 135 Cyclin-afhængig kinase 1 nukleare lokalisering signal kinase assay fosforylering massespektrometri analytiske gel filtrering karyopherin alpha CENP-F G2 fase cellecyklus kinase affinitet rensning
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