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Forbedring af engraftment af humane inducerede pluripotente stamcelle afledte Kardiomyocytter via en forbigående hæmning af Rho Kinaseaktivitet
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1Department of Biomedical Engineering, School of Medicine, School of Engineering,University of Alabama at Birmingham, 2Division of Cardiovascular Diseases, School of Medicine, School of Engineering,University of Alabama at Birmingham, 3Department of Cardiac Surgery,The Second Xiangya Hospital of Central South University
Chapters
- 00:04Title
- 01:13hiPSC-CMs Purification, Small Molecule Pre-treatment, Myocardial Infarction, and Cell Transplantation
- 03:42Calcium Transient and Contractility Recording
- 05:21Results: Increased Engraftment of Transplanted hiPSC-CM Preconditioned with the ROCK Inhibitor Y-27632 in a Murine MI Model
- 07:11Conclusion
I denne protokol viser og uddyber vi, hvordan man bruger menneskeskabte pluripotente stamceller til differentiering og rensning af kardiomyocytter, og yderligere om, hvordan man kan forbedre sin transplantations effektivitet med Rho-associeret proteinkinasehæmmer forbehandling i en mus myokardieinfarkt model.
