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Journal / Genetics / Biotin-baserte Pulldown analysen å validere mRNA…
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JoVE Journal Genetics
Biotin-baserte Pulldown analysen å validere mRNA mål av mobilnettet miRNAs
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Biotin-baserte Pulldown analysen å validere mRNA mål av mobilnettet miRNAs

Article DOI: 10.3791/57786-v • 11:00 min • June 12th, 2018
June 12th, 2018 •
1,2,3, 2, 2, 2
1School of Biotechnology, Kalinga Institute of Industrial Technology University, 21. Center for AIDS Health Disparities Research Department of Biochemistry and Cancer Biology, Meharry Medical College, 3Kalinga Institute of Industrial Technology University

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Denne rapporten beskriver en rask og pålitelig metode for å validere mRNA mål av mobilnettet miRNAs. Metoden bruker syntetiske biotinylated låst nukleinsyre LNA-baserte miRNA etterligner for å fange mål mRNA. Deretter streptavidin-belagt magnetiske perler er ansatt å pulldown målet mRNA for kvantifisering av qPCR polymerasekjedereaksjons.

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Genetikk problemet 136 miRNA mRNA LNA biotinylation PCR 3' UTR
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