Journal
/
Genetics
/
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal Genetics
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

DOI:
10.3791/60906-v

•

05:45 min

•

March 11, 2020

•

, , ,
1ErasmusMC, Department of Viroscience, WHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fever Reference and Research,Erasmus University Medical Center

Chapters

  • 00:04Introduction
  • 00:59Nanopore Data Consensus Sequence Generation
  • 02:33Nanopore Sequencing Error Profile Compensation
  • 03:54Results: Representative Nanopore Sequencing Error Rate Overview
  • 04:58Conclusion

Summary

Automatic Translation

Automatic Translation

We previously validated a protocol for amplicon-based whole genome Usutu virus (USUV) sequencing on a nanopore sequencing platform. Here, we describe the methods used in more detail and determine the error rate of the nanopore R10 flow cell.

Tags

Artificial IntelligenceAI Writing AssistantsCopywritingContent GenerationText-based ContentContent CreationAI-assisted WritingWriter’s BlockContent EfficiencyReal-time SequencingVirus Genetic SequencingNanopore SequencingUsutu VirusError RateRead CoverageSequence DataConsensus SequenceReference-based AlignmentMinimap2SamtoolsKMA

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/60906?article_type=v

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
check_url/60906?article_type=v

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis
check_url/60906?article_type=v

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
check_url/60906?article_type=v

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System
check_url/60906?article_type=v

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
check_url/60906?article_type=v

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen
check_url/60906?article_type=v

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
check_url/60906?article_type=v

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
check_url/60906?article_type=v

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
check_url/60906?article_type=v

Identification of Circular RNAs using RNA Sequencing

Read Article