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JoVE Journal Genetics
En kombinasjon encellede tilnærming som karakteriserer den molekylære og Immunophenotypic heterogenitet menneskelige stammen og stamfar bestander
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En kombinasjon encellede tilnærming som karakteriserer den molekylære og Immunophenotypic heterogenitet menneskelige stammen og stamfar bestander

Article DOI: 10.3791/57831-v • 09:34 min • October 25th, 2018
October 25th, 2018 •
1, 1, 1, 1
1Division of Molecular Hematology, Lund Stem Cell Center, Lund University

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Bulk genet uttrykk målinger Sky enkeltcelle forskjeller i ulike celle populasjoner. Her beskriver vi en protokoll for hvordan encellede gene expression analyse og indeks sortering av Florescence aktivert celle sortering (FACS) kan kombineres for å avgrense heterogenitet og immunophenotypically karakterisere molecularly ulike celle populasjoner.

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Genetikk problemet 140 heterogenitet encellede RT-qPCR FACS indeks-sortering immunophenotypic karakteristikk gene expression analyse
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