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Chapters
- 00:04Title
- 00:56Cloning the Recipient Vector to Include a Tissue-specific Promoter
- 03:48Cloning the Sponge
- 04:55Generation of Stable H9c2 miR-181-sponge Expressing Cells
- 05:53Synthesis and Purification of Sponge-nanoparticles (miR-181-sponge Nanoparticles)
- 07:01Results: Validation of Nanonvector Delivery of the miR-181-sponge and its Effect on miR-181c Expression
- 07:58Conclusion
Vev-spesifikke microRNA hemming er en teknologi som er underutviklet i feltet microRNA. Her beskriver vi en protokoll for vellykket hemme miR-181 microRNA familien i myoblast celler fra hjertet. Nanovector teknologi brukes til å levere en microRNA svamp som viser betydelig i vivo cardio-spesifikke miR-181 med familien hemming.
