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In Vivo Nanovector levering af en hjerte-specifikke MicroRNA-svamp
JoVE Journal
Biology
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JoVE Journal Biology
In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
DOI:

09:53 min

June 15, 2018

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Chapters

  • 00:04Title
  • 00:56Cloning the Recipient Vector to Include a Tissue-specific Promoter
  • 03:48Cloning the Sponge
  • 04:55Generation of Stable H9c2 miR-181-sponge Expressing Cells
  • 05:53Synthesis and Purification of Sponge-nanoparticles (miR-181-sponge Nanoparticles)
  • 07:01Results: Validation of Nanonvector Delivery of the miR-181-sponge and its Effect on miR-181c Expression
  • 07:58Conclusion

Summary

Automatic Translation

Væv-specifikke microRNA hæmning er en teknologi, der er underudviklede i feltet microRNA. Heri, beskriver vi en protokol for at kunne hæmme familien miR-181 microRNA i myoblast celler fra hjertet. Nanovector teknologi bruges til at levere en mikroRNA svamp, der viser betydelig i vivo cardio-specifikke miR-181 familie hæmning.

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