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一种基于管尖的液滴微流化平台种子细胞的方法
JoVE Journal
Engineering
This content is Free Access.
JoVE Journal
Engineering
A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms
Please note that all translations are automatically generated.
Click here for the English version.
一种基于管尖的液滴微流化平台种子细胞的方法
DOI:
10.3791/57848-v
•
06:50 min
•
February 11, 2019
•
Nidhi Sinha*
1
,
Nikita Subedi*
1
,
Florian Wimmers*
2
,
Melf Soennichsen
,
Jurjen Tel
1
Department of Biomedical Engineering and Institute for Complex Molecular Systems, Laboratory of Immunoengineering
,
Eindhoven University of Technology
,
2
Institute for Immunity, Transplantation, and Infection, Beckman Center
,
Stanford University
Chapters
00:04
Title
00:42
Lossless Cell Encapsulation
03:04
Emulsion Breaking and Cell Retrieval for Flow Cytometric Analysis
04:30
Results: Representative Cell Encapsulation in Droplets
05:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文提出了一种利用管道尖头对液滴微流体装置播种稀缺细胞群体的方案, 以提供更高的液滴细胞封装效率。
Tags
Keywords: Pipette-tip
Cell Seeding
Droplet Microfluidics
Systems Immunology
Cellular Heterogeneity
Communication
Rare Cells
Encapsulation
Aqueous Droplet Generation
Surfactant
Fluorinated Oil
Mineral Oil
PDMS
Syringe Pump
Sample Solution
Oil Phase
RPMI Medium
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