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JoVE Journal
Biochemistry

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用质谱和电镜分析 Biolayer 干涉传感器上的动态蛋白复合物的组装和释放
 

用质谱和电镜分析 Biolayer 干涉传感器上的动态蛋白复合物的组装和释放

Article doi: 10.3791/57902
August 6th, 2018 Usage Statistics

Summary August 6th, 2018

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在这里, 我们提出了一个协议, 以监测炭疽毒素的组装和拆卸使用 biolayer 干涉测量 (BLI)。在生物传感器表面组装/拆卸后, 通过电子显微镜和质谱法, 从表面释放出大量的蛋白质复合物, 用于可视化和识别复合物的成分。

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