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使用液体色谱加上串联质谱法的 糖核 酸菌的定量元细胞学
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
DOI:

07:25 min

January 05, 2021

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Chapters

  • 00:04Introduction
  • 00:42Cell Transfer and Culture
  • 01:21Metabolite Extraction
  • 02:44Extracted Metabolite Separation and Analysis
  • 04:04Membrane Integrity Assessment
  • 05:24Results: Representative Metabolic Intermediate and Product Identification and Quantification
  • 06:50Conclusion

Summary

Automatic Translation

我们提出了一个协议,用于识别和量化主要类别的水溶性代谢物在酵母 糖体中。所述方法通用、坚固且灵敏。它允许结构异构体和立体形式的水溶性代谢物彼此分离。

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