Journal
/
Biochemistry
/
使用液体色谱加上串联质谱法的 糖核 酸菌的定量元细胞学
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Please note that all translations are automatically generated. Click here for the English version.

使用液体色谱加上串联质谱法的 糖核 酸菌的定量元细胞学

DOI:
10.3791/62061-v

•

07:25 min

•

January 05, 2021

•

, ,
1Department of Biology,Concordia University, 2Centre for Biological Applications of Mass Spectrometry,Concordia University

Chapters

  • 00:04Introduction
  • 00:42Cell Transfer and Culture
  • 01:21Metabolite Extraction
  • 02:44Extracted Metabolite Separation and Analysis
  • 04:04Membrane Integrity Assessment
  • 05:24Results: Representative Metabolic Intermediate and Product Identification and Quantification
  • 06:50Conclusion

Summary

Automatic Translation

Automatic Translation

我们提出了一个协议，用于识别和量化主要类别的水溶性代谢物在酵母 糖体中。所述方法通用、坚固且灵敏。它允许结构异构体和立体形式的水溶性代谢物彼此分离。

Tags

Quantitative MetabolomicsSaccharomyces CerevisiaeLiquid ChromatographyTandem Mass SpectrometryQuenchingMetabolite ExtractionChloroformMethanolGlass BeadsVortexAqueous PhaseAcetonitrileLiquid Chromatography-tandem Mass Spectrometry

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

时间分辨电喷雾电离氢氘交换质谱用于研究蛋白质结构与动态

解决由蓝变性PAGE和蛋白质相关剖析亲和纯化的蛋白复合物

对于代谢物分析基于法 - 串联液相色谱 - 质谱法<em>金黄色葡萄球菌</em

脂滴隔离定量质谱分析

用等压标记、广泛的液相色谱法、质谱法和软件辅助定量技术进行深层蛋白质组分析

通过亲和色谱与西布洛特或质谱结合的亲致色谱,识别磷酸磷酸磷酸磷酸醇或磷酸蛋白

通过反相液相-质谱法对无细胞蛋白合成代谢的绝对定量

使用液色谱结合串联质谱法对糖皮质酶细胞脂质的定量分析

无目标液相色谱-质量光谱学-小麦粒基代谢代谢学分析

肽聚糖的液相色谱质谱和生物信息学半定量分析

Read Article