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使用液色谱结合串联质谱法对糖皮质酶细胞脂质的定量分析
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Biochemistry
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JoVE Journal Biochemistry
Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
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使用液色谱结合串联质谱法对糖皮质酶细胞脂质的定量分析

DOI:
10.3791/60616-v

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08:56 min

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March 08, 2020

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, , ,
1Department of Biology,Concordia University, 2Department of Chemistry and Biochemistry, Centre for Biological Applications of Mass Spectrometry,Concordia University

Chapters

  • 00:05Introduction
  • 00:34Yeast Culture and Lipid Extraction
  • 03:55Extracted Lipid Separation
  • 05:26Mass Spectrometric Analysis and Lipid Class Identification and Quantification
  • 06:53Results: Representative Quantitative Analysis of Saccharomyces Cerevisiae Cellular Lipidomes
  • 08:19Conclusion

Summary

Automatic Translation

Automatic Translation

我们提出一个协议，使用液体色谱结合串联质谱法识别和量化主要细胞脂在糖体质酶。所述的酵母细胞内主要脂质类定量评估的方法用途广泛、健壮且敏感。

Tags

LipidomeSaccharomyces CerevisiaeLiquid ChromatographyTandem Mass SpectrometryLipid ExtractionYeast CultureInternal Lipid StandardsQuantitative Analysis

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