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Journal / Genetics / 哺乳动物系统 CRISPR 指南 RNA 克隆
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Genetics

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JoVE Journal Genetics
哺乳动物系统 CRISPR 指南 RNA 克隆
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哺乳动物系统 CRISPR 指南 RNA 克隆

Article DOI: 10.3791/57998-v • 06:48 min • October 2nd, 2018
October 2nd, 2018 •
*1,2, *1,2,3, 1,2, 1,2, 1, 1, 1,4,5,6,7, 1,2
1Wyss Institute for Biologically Inspired Engineering, Harvard University, 2Department of Genetics, Harvard Medical School, 3Department of Pathology, Massachusetts General Hospital, 4Institute for Medical Engineering & Science, Massachusetts Institute of Technology, 5Synthetic Biology Center, Massachusetts Institute of Technology, 6Department of Biological Engineering, Massachusetts Institute of Technology, 7Broad Institute
* These authors contributed equally

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这里概述了一种简单、高效、经济高效的 sgRNA 克隆方法。

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遗传学 问题 140 CRISPR 伴生的蛋白质 9 CRISPR gRNA 指南 RNA sgRNA pSB700
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