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沙飞（木薯）胚胎微注射为CRISPR/Cas9穆塔赫内西
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Genetics
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JoVE Journal Genetics
Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis
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沙飞（木薯）胚胎微注射为CRISPR/Cas9穆塔赫内西

DOI:
10.3791/61924-v

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05:44 min

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November 17, 2020

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, , , , ,
1Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases,National Institutes of Health, 2University of Maryland Insect Transformation Facility,The Institute for Bioscience and Biotechnology Research

Chapters

  • 00:04Introduction
  • 00:38Embryo Collection and Micromanipulation
  • 02:03Embryo Injections
  • 03:59Results: Representative Identification of Mosaic G0 Adults and Mutations of Interest
  • 05:07Conclusion

Summary

Automatic Translation

Automatic Translation

该协议详细介绍了CRISPR/Cas9在沙蝇中靶向诱变的步骤：胚胎采集、注射、昆虫饲养和识别，以及兴趣突变的选择。

Tags

Sand FlyPhlebotomus PapatasiEmbryo MicroinjectionCRISPR/Cas9MutagenesisInsect Genome EditingBlood FeedingEgg-layingFilter PaperMicroscopeInjection NeedlePneumatic Injection Controller

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