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JoVE Journal Developmental Biology
Sinaptik Microcircuit modelleme 3D Cocultures Astrocytes ve Neurons insan Pluripotent kök hücre ile
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Sinaptik Microcircuit modelleme 3D Cocultures Astrocytes ve Neurons insan Pluripotent kök hücre ile

Article DOI: 10.3791/58034-v • 08:48 min • August 16th, 2018
August 16th, 2018 •
1, 1, 1
1Center for Neuroregeneration, Department of Neurosurgery, Houston Methodist Research Institute

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Bu iletişim kuralı, ayrışmış birleştirerek insan pluripotent kök hücre elde edilen nöronlar için tekrarlanabilir bir yöntemi açıklamak için amacımız ve astrocytes 3D içine birlikte cocultures, bunlar yuvar ücretsiz kayan koşulları ve daha sonra korumak küre immunoanalysis ve multielectrode dizi kayıtları ile küre sinaptik devre etkinliğin ölçülmesi.

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Gelişim biyolojisi sayı 138 kök hücre nöronlar Astrocytes sinapslarda Organoid Coculture biyoreaktör Multielectrode dizi
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