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Journal / Genetics / Singola goccia digitale Polymerase Chain Reaction …
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JoVE Journal Genetics
Singola goccia digitale Polymerase Chain Reaction per rilevazione completa e simultanea delle mutazioni nelle regioni di Hotspot
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Singola goccia digitale Polymerase Chain Reaction per rilevazione completa e simultanea delle mutazioni nelle regioni di Hotspot

Article DOI: 10.3791/58051-v • 08:23 min • September 25th, 2018
September 25th, 2018 •
1,2, 1, 1, 1,3,4, 1,3,5, 1,6, 1
1Circulating Tumor Biomarkers Laboratory, SiRIC, Translational Research Department, Institut Curie, PSL Research University, 2CNRS UMR144, Institut Curie, PSL Research University, 3Department of Medical Oncology, Institut Curie, PSL Research University, 4University Versailles Saint-Quentin-en-Yvelines, 5University Paris Descartes, 6Inserm U830, Institut Curie, PSL Research University

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Qui, presentiamo un protocollo per quantificare con precisione le alterazioni genetiche multiple di una regione di destinazione in una singola reazione usando drop-off ddPCR e un paio di idrolisi sonde uniche.

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Genetica problema 139 drop-off ddPCR mutazioni di Hotspot cancro tumore del DNA diagnostica di circolazione
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