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单液滴数字聚合酶链反应对热点地区突变的综合和同步检测
JoVE Journal
Genetics
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JoVE Journal Genetics
Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
DOI:

08:23 min

September 25, 2018

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Chapters

  • 00:04Title
  • 00:48Blood Collection and Plasma Storage
  • 02:04cfDNA Extraction
  • 04:45ddPCR Mix Preparation
  • 05:46Droplet Generation
  • 06:25PCR Amplification
  • 07:06Results: KRAS and EGFR Drop-off ddPCR Assays
  • 07:50Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个协议, 以准确地量化一个目标区域的多基因变化, 在单一的反应使用下拉 ddPCR 和一个独特的对水解探针。

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