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Journal
/
Genetics
/
单液滴数字聚合酶链反应对热点地区突变的综合和同步检测
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Genetics
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Genetics
Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
Please note that all translations are automatically generated.
Click here for the English version.
单液滴数字聚合酶链反应对热点地区突变的综合和同步检测
DOI:
10.3791/58051-v
•
08:23 min
•
September 25, 2018
•
Charles Decraene
2
,
Amanda Bortolini Silveira
,
Marc Michel
,
François-Clément Bidard
3,4
,
Jean-Yves Pierga
3,5
,
Marc-Henri Stern
6
,
Charlotte Proudhon
1
Circulating Tumor Biomarkers Laboratory, SiRIC, Translational Research Department
,
Institut Curie, PSL Research University
,
2
CNRS UMR144
,
Institut Curie, PSL Research University
,
3
Department of Medical Oncology
,
Institut Curie, PSL Research University
,
4
University Versailles Saint-Quentin-en-Yvelines
,
5
University Paris Descartes
,
6
Inserm U830
,
Institut Curie, PSL Research University
Chapters
00:04
Title
00:48
Blood Collection and Plasma Storage
02:04
cfDNA Extraction
04:45
ddPCR Mix Preparation
05:46
Droplet Generation
06:25
PCR Amplification
07:06
Results:
KRAS
and
EGFR
Drop-off ddPCR Assays
07:50
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个协议, 以准确地量化一个目标区域的多基因变化, 在单一的反应使用下拉 ddPCR 和一个独特的对水解探针。
Tags
Single Droplet Digital PCR
Circulating Tumor DNA
Mutation Detection
Hotspot Regions
Cancer Diagnosis
Absolute Quantification
Molecular Analysis
QPCR Alternative
Plasma Extraction
Proteinase K
Lysis Buffer
Silica Membrane
Vacuum Pump
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