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En høj overførselshastighed Cre-Lox aktiveret Viral membran Fusion analyse for at identificere hæmmere af HIV-1 Viral membran Fusion
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Immunology and Infection
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JoVE Journal Immunology and Infection
A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion

En høj overførselshastighed Cre-Lox aktiveret Viral membran Fusion analyse for at identificere hæmmere af HIV-1 Viral membran Fusion

DOI:
10.3791/58074-v

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07:22 min

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August 14, 2018

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, , , , ,
1Division of Infectious Diseases, Department of Medicine,Icahn School of Medicine at Mount Sinai, Immunology Institute, 2Department of Biology,New Jersey City University

Chapters

  • 00:04Title
  • 00:54Preparation of Target Cells for Cell-to-Cell Virus Transmission
  • 02:11Preparation of Donor Cells
  • 03:56Co-culture
  • 05:06Fluorescence-activated Cell Sorting
  • 05:35Results: Identification of a Novel HIV-1 Viral Membrane Fusion Inhibitor
  • 06:49Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver en celle-baseret analyse for at rapportere om HIV-1 fusion via udtryk for grøn fluorescerende proteiner kan påvises ved flow flowcytometri eller Fluorescens mikroskopi. Det kan bruges til at teste hæmmere af viral entry (specielt på trinnet fusion) i celle-fri og celle til celle infektion systemer.

Tags

Cre-LoxViral Membrane FusionHIV-1Drug DiscoveryHigh-throughput ScreeningHIV Fusion InhibitorsBiosafetyJurkat RG Reporter CellsPuromycinCell-to-cell Virus TransmissionGag-iCre DNAElectroporationFicoll

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