Medicine
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用膝关节关节仪评价大鼠膝关节屈曲收缩的特定组织贡献
Chapters
Summary November 9th, 2018
Please note that all translations are automatically generated.
Click here for the English version.
该方案的目的是测量大鼠膝关节运动的扩展范围。各种疾病增加膝关节僵硬的影响和治疗的有效性可以量化。
Transcript
此方法可以帮助回答肌肉骨骼领域的关键问题，例如使用大鼠模型干预对膝关节运动范围的影响。该技术的主要优点是，它提供了一种精确、可重复、用户独立的测量鼠膝关节延伸的方法。首先使用剪刀和钳子去污的安乐死大鼠的下肢，并去除皮肤从底层筋膜。
然后，将身体放在其一侧，实验腿朝上。将股骨放在集成在关节计安装阶段的凹槽金属夹中。用精密螺丝刀通过肌肉打孔，将夹子端放在更大的肌管上，以固定股骨。
然后，在关节计旋转中心上调整横向股骨。将可移动臂放在腿部后面两个直立柱的位置，在电机激活后，将膝盖推入被动延伸。使用六角钥匙拧紧底座夹紧，直到固定。
确保使用螺丝刀将相机正确安装在测量仪上。检查相机是否位于手动对焦上，然后将相机对焦在女性对焦上。根据所测试的膝关节运动范围方向和大鼠的位置，顺时针或逆时针设置关节计的方向。
通过同时按下电源和启动按钮激活关节计电机。关节计电机将以 6.6 rpm 的速度移动，然后在达到第一个预设扭矩时停止 2.1 秒。当达到第一个扭矩时，相应的 LED 指示灯将点亮，数码相机将自动拍摄膝盖的照片。
拍摄照片后，关节计将继续到下一个更高的预设扭矩。一旦应用了四个扭矩，关节计将停止。一旦大鼠定位在关节仪上并启动测试，测试单膝的总时间约为 18.8 秒。
要分离收缩的关节原生或非肌肉成分，请重置扭矩序列以释放施加在腿部的力，然后对后侧关节肌肉进行肌切除术。解剖肌肉近向膝盖关节，以确保胶囊不切割。在肌切除术前和之后测试两条腿，并解剖出任何所需的组织后，按照机构协议处理动物尸体和所有生物危害物质，并清洁关节计。
打开包含安装在图像 J 中关节仪上的相机拍摄的数字图像的文件。从主工具栏中选择角度工具，通过绘制股骨夹中间到侧侧凹面的股骨线和从侧股股骨到侧毛的股骨线到侧侧毛角，跟踪股骨-骨角。使用测量工具，单击"分析"然后"测量"以显示上面绘制的两条线产生的计算角度。
使用零度约定表示完全扩展。这图显示，与非固定反向肢体相比，7只大鼠在16周固定后最大膝盖延长。对于固定膝盖，最大延长能力比反面的降低。
在肌切除术之后，实验膝和反向膝的最大延长能力增强，然而，实验膝盖继续表现出弯曲收缩。在尝试此程序时，确保股骨夹安全良好，以避免在测试期间膝盖运动，这一点很重要。按照这个程序，其他方法，如组织分析膝盖，关节结构可以回答与组织和细胞变化有关的其他问题，发生在膝盖关节，以影响其运动范围。
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