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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolering og identifikation af ekstravaskulære immunceller af hjertet
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Isolering og identifikation af ekstravaskulære immunceller af hjertet

Article DOI: 10.3791/58114-v • 08:24 min • August 23rd, 2018
August 23rd, 2018 •
1,2, 1,2,3,4,5, 1,2
1Toronto General Hospital Research Institute, University Health Network (UHN), 2Dept of Laboratory Medicine and Pathobiology, University of Toronto, 3Dept of Immunology, University of Toronto, 4Peter Munk Cardiac Centre, 5Ted Rogers Centre for Heart Research

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Denne protokol udgør en enkel og effektiv metode til at isolere, identificere og kvantificere immunceller bosat i myokardiet af mus under steady state eller betændelse. Protokollen kombinerer enzymatiske og mekaniske fordøjelsen for generation af en enkelt cellesuspension, der yderligere kan analyseres ved flowcytometri.

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Immunologi og infektion spørgsmålet 138 myokardiet immun celler makrofager dendritiske celler celle dyrkning væv fordøjelsen
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