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Immunology and Infection
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Karakterisering af immunceller og proinflammatoriske mediatorer i lungemiljøet
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Immunology and Infection
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JoVE Journal Immunology and Infection
Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment

Karakterisering af immunceller og proinflammatoriske mediatorer i lungemiljøet

DOI:
10.3791/61359-v

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09:00 min

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June 24, 2020

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, , ,
1Department of Microbiology, Immunology, and Cell Biology,West Virginia University, 2Department of Neuroscience,West Virginia University, 3Rockefeller Neuroscience Institute,West Virginia University

Chapters

  • 00:04Introduction
  • 00:45Lung Tissue Harvest
  • 02:50Lung Tissue Homogenization
  • 03:44Lung Tissue Dissociation and Single Cell Isolation
  • 05:21Flow Cytometric Lung Immune Cell Niche Analysis
  • 06:27Results: Representative Immune Cell Population and Chemokine Expression Characterization
  • 08:35Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver brugen af flowcytometri til at identificere ændringerne i immuncellesammensætning, cytokinprofil og kemokinprofil i lungemiljøet efter forbigående mellem cerebral arterieokklusion, en murinmodel af iskæmisk slagtilfælde.

Tags

Immune CellsProinflammatory MediatorsPulmonary EnvironmentIschemic StrokeFlow Cytometric AnalysisLung Immune Cell TypesCytokinesChemokinesWhole Body Transcardial PerfusionMultiplex Bead ArraysHomogenization BufferLung Cell Medium

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