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Differentiering funktionelle roller af genekspression fra immun og ikke-immun celler i Mouse Colitis af knoglemarvstransplantation
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Immunology and Infection
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JoVE Journal Immunology and Infection
Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

Differentiering funktionelle roller af genekspression fra immun og ikke-immun celler i Mouse Colitis af knoglemarvstransplantation

DOI:
10.3791/4208-v

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14:43 min

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October 01, 2012

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, , , ,
1Center for Inflammatory Bowel Diseases, Division of Digestive Diseases, David Geffen School of Medicine,The University of California Los Angeles, Los Angeles

Chapters

  • 00:05Title
  • 01:26Experimental Setup
  • 02:39Donor Bone Marrow Extraction
  • 05:49Infusion to Recipient Mice
  • 07:19Induction of Colitis and Evaluation of Colitis
  • 08:55Quality Inspection of Bone Marrow Transplantation by Flow Cytometry
  • 11:59Transplantation Results
  • 14:21Conclusion

Summary

Automatic Translation

Automatic Translation

Knoglemarvstransplantation tilvejebringer en måde at ændre genotype af knoglemarv-afledte celler. Hvis genet af interesse udtrykkes i både knoglemarvsafledte celler og ikke-knoglemarvsafledte celler, kan knoglemarvstransplantation ændre knoglemarv afledte celler til en anden genotype uden at ændre den ikke-knoglemarvsafledte celler genotype.

Tags

Gene ExpressionImmune CellsNon-immune CellsMouse ColitisBone Marrow TransplantationGene-of-interestKnockout MiceGenotypeIrradiationDonor Bone MarrowRecipient MiceWild-type MiceCD Molecules (CD45.1 And CD45.2)

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