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En In Vivo musemodel foranstaltning naive CD4 T-celle aktivering, spredning og Th1 differentiering induceret af knoglemarv-afledte dendritiske celler
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Chapters
- 00:04Title
- 00:46Mouse Bone Marrow (BM) and Secondary Lymphoid Tissue Cell Isolation
- 03:40In Vivo Activation, Proliferation, and Th1 Cell Differentiation Assay
- 06:48Results: Representative BM-derived Dentritic Cell-induced Naïve CD4+ T Cell Activation, Proliferation, and Th1 Differentiation
- 07:39Conclusion
Vi præsenterer her, en protokol til i vivo bestemmelse af naive CD4 T celle (T-celle) aktivering, spredning og Th1 differentiering induceret af GM-CSF knoglemarv (BM)-afledt dendritiske celler (DCs). Derudover beskriver denne protokol BM og T-celle isolation, DC generation og DC og T-celle adoptiv overførsel.
