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En In Vivo musmodell åtgärd naiva CD4 T-cellsaktivering, spridning och Th1 differentiering induceras av benmärgen-derived dendritiska celler
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
An In Vivo Mouse Model to Measure Naïve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells
DOI:

08:39 min

August 22, 2018

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Chapters

  • 00:04Title
  • 00:46Mouse Bone Marrow (BM) and Secondary Lymphoid Tissue Cell Isolation
  • 03:40In Vivo Activation, Proliferation, and Th1 Cell Differentiation Assay
  • 06:48Results: Representative BM-derived Dentritic Cell-induced Naïve CD4+ T Cell Activation, Proliferation, and Th1 Differentiation
  • 07:39Conclusion

Summary

Automatic Translation

Här presenterar vi ett protokoll för i vivo bestämning av naiva CD4 T cell (T cell) aktivering och spridning Th1 differentiering induceras av GM-CSF benmärg (BM)-härledda dendritiska celler (DCs). Dessutom beskriver det här protokollet BM och T-cell isolering, DC generation och DC och T-cell överföring.

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