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Autometallography 在鲸目组织中的定位和半定量银的应用
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JoVE Journal
Environment
Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues
Please note that all translations are automatically generated.
Click here for the English version.
Autometallography 在鲸目组织中的定位和半定量银的应用
DOI:
10.3791/58232-v
•
07:05 min
•
October 04, 2018
•
Wen-Ta Li
,
Bang-Yeh Liou
,
Wei-Cheng Yang
,
Meng-Hsien Chen
,
Hui-Wen Chang
2
,
Hue-Ying Chiou
,
Victor Fei Pang
2
,
Chian-Ren Jeng
2
1
Graduate Institute of Molecular and Comparative Pathobiology
,
National Taiwan University
,
2
School of Veterinary Medicine
,
National Taiwan University
,
3
Department of Oceanography and Asia-Pacific Ocean Research Center
,
National Sun Yat-sen University
,
4
Graduate Institute of Veterinary Pathobiology
,
National Chung Hsing University
Chapters
00:04
Title
00:57
Autometallography Analysis Tissue Sample Preparation
03:48
Semi-Quantitative AMG Analysis and Cetacean Histological Ag Assay (CHAA) by Regression
05:54
Results: Representative Histological Images of AMG Positive Signals in the Liver and Kidney Tissues of Stranded Cetaceans
06:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文提出了 autometallography 在鲸鱼肝和肾脏组织中定位银的协议。此外, 还开发了一种新的检测方法, 名为鲸类组织学 ag 测定 (CHAA), 用以估计这些组织中的银浓度。
Tags
Autometallography
Silver
Environmental Toxicology
Heavy Metals
Liver
Kidney
Formalin Fixation
Paraffin Embedding
Microtome
De-paraffination
Hydration
PBS
Triton X-100
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