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Genetics
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可抽税抗生素耐药
盒 P1 转导制备大肠杆菌中的基因缺失
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Genetics
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JoVE Journal
Genetics
Producing Gene Deletions in
Escherichia coli
by P1 Transduction with Excisable Antibiotic Resistance Cassettes
Please note that all translations are automatically generated.
Click here for the English version.
可抽税抗生素耐药
盒 P1 转导制备大肠杆菌中的基因缺失
DOI:
10.3791/58267-v
•
08:13 min
•
September 01, 2018
•
Athanasios Saragliadis
,
Thomas Trunk
,
Jack C. Leo
1
Evolution and Genetics, Department of Biosciences
,
University of Oslo
Chapters
00:04
Title
00:28
Phage Lysate Preparation
02:31
P1 Transduction
04:30
Kanamycin (Kan) Cassette Excision
06:03
Results: TAA Expression by a
tamA
Deletion Strain
07:38
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了使用预先存在的抗生素耐药性-盒式删除结构作为一个基础, 使删除突变体在其他
大肠杆菌
菌株。这种删除突变可以调动和插入到相应的受体菌株的位置使用 P1 噬菌体转导。
Tags
P1 Transduction
Gene Deletion
Escherichia Coli
Antibiotic Resistance Cassette
Knockout Mutant
Phage
Bacterial Culture
Lysogeny Broth
Centrifugation
Chloroform
Sodium Citrate
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