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种系线虫与胸苷模拟教育的细胞周期分析
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Developmental Biology
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JoVE Journal Developmental Biology
Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU
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种系线虫与胸苷模拟教育的细胞周期分析

DOI:
10.3791/58339-v

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06:58 min

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October 22, 2018

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, , ,
1Department of Developmental Biology,Washington University School of Medicine, St. Louis, Missouri, 2Department of Genetics,Washington University School of Medicine, St. Louis, Missouri

Chapters

  • 00:04Title
  • 00:505-ethynyl-2′-deoxyuridine (EdU)-labeled Bacteria Preparation and C. elegans Feeding
  • 02:46Germline Dissection, Fixation, Rehydration, and EdU Click Reaction/Detection
  • 05:09Results: Representative C. elegans Cell Cycle Analyses
  • 06:20Conclusion

Summary

Automatic Translation

Automatic Translation

本文描述了一种基于成像的方法, 可用于识别 S 相和分析种系中的细胞周期动力学. 此方法不需要转基因, 并且与免疫荧光染色兼容。

Tags

Cell Cycle AnalysisC. ElegansGermlineEdUThymidine AnalogImmunofluorescent StainingGerm Line Stem CellCell Cycle DynamicsAgingNutritionAltered GenotypesIdiodisprepationHematoxylin StainingSterile TechniqueM9 BufferE. ColiEdU SupplementationCentrifugationNematode Growth MediumPBSDissectionFixation

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