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Cellecyklus analyse i C. elegans kønscelleoverførsel med Thymidine Analog EdU
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU
DOI:

06:58 min

October 22, 2018

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Chapters

  • 00:04Title
  • 00:505-ethynyl-2′-deoxyuridine (EdU)-labeled Bacteria Preparation and C. elegans Feeding
  • 02:46Germline Dissection, Fixation, Rehydration, and EdU Click Reaction/Detection
  • 05:09Results: Representative C. elegans Cell Cycle Analyses
  • 06:20Conclusion

Summary

Automatic Translation

En billeddannelse-baseret metode er beskrevet som kan bruges til at identificere S-fase og analysere cellecyklus dynamics i C. elegans hermafrodit kønscelleoverførsel ved hjælp af thymidine analog EdU. Denne metode kræver ingen transgener og er kompatibel med immunfluorescent farvning.

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